can anyone please send me the exact and proved protocol of zymography.i tried to do zymography many times with different protocol but i am not getting the clear band as mentioned in many papers,my protein does not seem to run through the gel,so i get clear zone at the top of gel.
you can send at aquarian912@gmail.com

Zymography has been discussed in another forum. Take a look at the protocols posted here:

http://www.scientistsolutions.com/t2676-gelatin+zymography.html