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Scientist Solutions is a life sciences discussion board run by scientists for scientists with the mission to connect every scientist in the world. This site is moderated by a team of scientific staff. It is our hope that Scientist Solutions will encourage the exchange of information between investigative researchers and thereby contribute to the advancement of Science.
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Academics
Online: 51   Welcome New Members: LEENA, marthaeu11, dylanorion, JAAFAR, harigopalarao, sonivijay07
Forum Topics Posts Last Post
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Discussion of anything scientific that does not fit into another category or forum. Examples range from “how to write the statistical symbol for mean” to “my TC incubator is contaminated.”
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Jobs
Post Job Openings for General Here
Life at Google, Google Workers Needed
Announcements
Official announcements from the Scientist Solutions team.
Obama and McCain: Both Pledge to Double NIH ...
Comments and Suggestions
Request features or recommendations
Science Debate 2008 Set for April 18th
Forum Suggestions
Recommend a new forum
Can u add Expert's Zone
Meeting Announcements
IAN 2008
Student Questions
discuss academic related topics
how do scientists get papers not available in ...
Miscellaneous
If you can't find the right place to post something, post it here!
Reporter Gene
301
438
You know you're a labrat when..
Oct 11, 2008 00:41 AM
Jokes
please keep it clean
You know you're a labrat when..
Quotes
Famous scientific quotes
Men build...
Anniversaries & Birthdays
famous scientists, major scientific achievements.
Emil Fischer
Milestones & Dedications
Supernova 1604, also known as Kepler's Supern ...
Strange Science Facts
solar eclipse shadows
Images & Movies
New Photo of Mercury
Lab Bratz
Science humor webcomic
Lab Bratz Episode #3
193
484
Determination of IPA by FTNIR
Oct 08, 2008 09:33 AM
Discussion chromatography (HPLC, FPLC, GC) spectroscopy, surface chemistry, thermal analysis, sample separation, extraction
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Meeting Announcements
Eastern Analytical Symposium
Protocols
Library of policies and practices.
Glossary of HPLC/LC Separation Terms
Sample Preparation
Sample analysis problems
Sample Preparation Users Poll
Chromatography
help on chromatographic techniques.
formic acid HPLC
Methods
Chemistry methods and problems, rather than instrumental
Good method for membrane protein western
Electron Spectroscopy/Microscopy
SEM, TEM, optical microscopy...
RTI International (www.rti.org) provides free ...
Electrophoresis (IEF, SDS-Page, 2D-Gel, Capillary, Native)
all electrophoretic techniques
Protein PEGylation
Laser Light Scattering
discuss light scattering techniques
Native protein molecular weight
Spectrometry (Atomic, Electron, Mass)
discuss spectroscopic methods
kindly give some points on flame photometry?? ...
Surface Chemistry
surface analysis, etc
about Erbium
Equipment
information on purchasing and selling
Determination of IPA by FTNIR
Post Ideas/Find Collaborator
Artificial skin model for teating of adhesion ...
Jobs
Analytical Chemistry openings
Analytical Chemist, Rockville, MD
217
682
Cell Culture contamination
Oct 08, 2008 09:22 AM
Discuss organization of the body and its functions including organs, tissues, electrophysiology, histology, fixation, immunohistochemistry, exercise, muscle, microdissection…
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Jobs
Anatomy and Physiology Jobs
Funded Graduate Position in Manitoba (Canada)
Cell Anatomy/Physiology
Cellular morphology
Cell Culture contamination
Comparative Anatomy and Physiology
Organization of the body, organs and tissues
Joint load
Electrophysiology
Electrical properties cells/tissues.
Help with stimulation electrodes
Human Anatomy/Physiology
Human organs/tissues/systems
May two women have children?
Equipment
Anatomical equipment
Santa Cruz c-fos antibody in brain tissue
Assay Development & Protocols
Protocols problems/design
elisa assay without a plate reader?
Post Ideas/Find Collaborator
A question about retrograde labeling
Meeting Announcements
The World Congress on Controversies in Cardio ...
85
106
Discussion about breeding, diseases, genetics, management, ethics, nutrition and physiology of farm, laboratory, companion animals…
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Jobs
Animal and Veterinary Science Jobs
Assistant Professor sought at University of W ...
Anatomy and Physiology
internal and external structure and the physical relationships between the body’s constituents
images/video of mitochondria
Animal Behavior
Development, functions, and evolution animal behavior
Preclinical CNS safety pharmacology of anti ...
Care and Ethics
How Can We Measure The Emotional States Of An ...
Animal Disease
Disease manifestation and treatments
Bovine Tuberculosis In Wildlife Threatens End ...
Ecology and Environmental Interactions
Relationships of animals to their environment.
Birds 'off the pace' with warming
Evolutionary Biology
Origin and descent of species
Introduction to Evolutionary Biology
Growth and Nutrition
Adding citrus juices or vitamin C to green te ...
Husbandry Technologies
breeding, feeding, housing and nutrition
Green Caffee Bean Extract Chlorogenic Acid on ...
Laboratory Animals
Morpholinos for adult animals
Marine Biology
organisms in the ocean or brackish water.
Fluorescent-Red Glowing Fish Found
Regulatory Issues
Regulatory Issues Surrounding the Use of Comp ...
Veterinary Science
medical, diagnostic, and therapeutics
Veterinary Pathology
Zoology & Developmental Zoology
Structure, physiology, development, and classification
Systems Biology: perturbing cellular gene reg ...
General Animal Science Discussion
Intradermal injection in mice
Meeting Announcements
BSAS Annual Conference 2009
189
529
scFv expression
Oct 07, 2008 23:05 PM
Discussion on antibodies techniques including polyclonal, monoclonal, peptide design, antisera, labeling, ELISA, fusion proteins, complement activation, isotypes, Fab, Fc, class switching…
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Presenting Prestige Antibodies
Jobs
Engineer - Automation/Instrumentation Job Ope ...
Antibody Fusion Proteins
soluble conjugates, blocking and activation reagents.
Ab / peptide immobilization
Epitope Mapping
immunogenic regions and antibody binding epitopes
T cell epitope mapping
Monoclonal Antibodies
clonal Abs
polyclonal antibody ..help
Recombinant Antibody Technology
Protein scaffolds for antibody mimics
Transgenic Animal Models
From TCR hybridomas to whole mouse genetic models...
transgenic mice/human antibodies
Equipment
ELISA plate readers, robots, flow cytometers, gel imagers
BSA as a stabilizing agent
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
scFv expression
Post Ideas/Find Collaborator
2007 Antibody Source Book from AbD Serotec
Meeting Announcements
Engineering the Next Generation of Antibodies
273
576
Concanavalin A sepharose
Oct 10, 2008 09:08 AM
Discussion about chemical processes in living organisms. Including enzymes, kinetics, biosynthesis, catalysis, nutrient metabolism, glycolysis, amino acid synthesis…
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Jobs
Biochemistry Jobs
Assistant Professor - Biochemistry
Chemical Carcinogenesis
chemical-induced mutagenesis, cancerous transformation of normal cells
Alcohol abuse affects millions
Chemiluminescence
luminescent detection assays
White spots?
Enzymology
kinetics, cofactors, co-enzymes, metabolic pathways
Enzyme Assays
Nuclear Magnetic Resonance (NMR)
quantum mechanical magnetic properties of an atom's nucleus.
'NMR On A Chip' Features NIST Magnetic Mini-s ...
Protein Structure/Modeling
three-dimensional modeling, de novo modeling, homology modeling
homology modeling using Swiss PBD
Equipment
What now?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Concanavalin A sepharose
Post Ideas/Find Collaborator
substrate preparation in krabbe disease(conce ...
Meeting Announcements
DNA damage: from causes to cures
87
233
Food Ethics (A New Frontier)
Oct 03, 2008 18:15 PM
Discussion on issues in bioethics including medical bioethics, ethics in the laboratory, plagiary, euthanasia, cloning, artificial life, organ donation, stem cell use…
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General Bioethics
medical bioethics, ethics in the laboratory work space, fraud, plagairism
Food Ethics (A New Frontier)
Jobs
Clinical Ethicist sought at UT MD Anderson
Cloning/Stem Cell Therapy
ethical issues concerning human or animal stem cell therapy and cloning
Going overseas for experimental stem cell the ...
Conflicts of Interest
evolution, religion, biotechnology or intellectual property
Talking about God at the Clinic
Genetically Modified Plants
long-term effects of genetically modified plants as a food and fuel source
Genetically Modified Plants
Medical Gene Therapy
gene therapy in the wake of clinical failures
Medical Gene Therapy Could be the Wave of the ...
Post Ideas/Find Collaborator
life science networking
Bioethics
Meeting Announcements
Bioethics Seminar Series at Purdue University
171
457
New Biology Search Engine
Oct 08, 2008 18:49 PM
Discussion bioinformatics including software development, programming, systems biology, computational models, semantic web, ontologies, data extraction, gene annotation…
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DNASTAR announces new Lasergene V8.0 Software ...
Jobs
Tips for finding a suitable post-doc position
Miscellaneous
Software from a user perspective
New Biology Search Engine
Systems Biology
Integrate different levels of information, wholistic rather than reductionist, computational models of biology.
ortholog detection by reciprocal BLAST
BiNS - Bioinformatics Student Network
ISCB Call for Applications
Semantic Web
Extension of the current web, data integration, ontology, knowledge management, web services...
wikiprofessional
Software Developers
What is the most common bioinformatics task?
Text and Data Mining
Extraction of information from unstructured, electronically accessible text resources
Curve pattern identification and categorizati ...
Workflows (Data Pipelines)
Workflows are series of programs that operate together to achieve goals such as the annotation of a genome. Discuss workflows that you've developed or that you would like to see built, and methods for building, running and sharing them (Perl, Taverna..)
Problem with web-Service for Taverna
Post Ideas/Find Collaborator
Need a collaborator? Read this first!
Meeting Announcements
International conference on applied bioengine ...
Sequence Analysis
DNA, Protein. Alignments (e.g. Blast), promoter identification, motif finding, transcription factor binding site prediction
DNA sequencing primers
About this forum
What is your preferred journal to publish in, ...
44
91
Tissue engineered bone
Oct 06, 2008 10:21 AM
Discussion on medical devices intended to interact with biological systems. Examples joint replacement, bone plates, cement, dental implants, heart valves, lens…
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Jobs
Biomedical Scientist opening
Biocompatibility
Ways to check for biocompatibility
Surface Modification
Material surface and biocompatibility
About C2C12
Biomaterials and Tissue Engineering
Hydrogels
Biomaterials and Medical Devices
Metals, biofilms, total hip replacement, artificial valves
Device to Manufacture Spider Silk
Biomaterials and Drug Delivery
Hiydrogels used for reparing human tissues
Meetings and Announcements
Tissue engineered bone
Post Ideas/Find Collaborators
Grant Ideas in Biomaterial Sciences
Assay Development & Protocols
This is a NEW Forum. Be 1st to post!
134
203
Faculty Position in Biophysics
Sep 26, 2008 14:07 PM
Interdisciplinary field applying techniques from the physical sciences to understanding biological structure and function at the molecular level.
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Jobs
Faculty Position in Biophysics
Crystallography/Crystallization
Arrangement of atoms in solids, uniform solution or melt
e-Crystallography course
Fluctuation Spectrometry
optical tweezer
Laser Confocal
High-resolution optical images, optical sectioning.
Nikon Instruments Launches Fully-Automated A1 ...
Raman Effect/FTIR
Killer Pulses Help Characterize Special Surfa ...
Scanning Probe Microscopy
Image surfaces at the nanometer scale
Nanotechnology: Femtogram-level Chemical Meas ...
Equipment
Electroporation cuvette gap size - Why?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Cell Biological Applications of Fluorescence ...
Post Ideas/Find Collaborator
Neutrinos: Tiny particles that fill the unive ...
Meeting Announcements
CELLULAR and MOLECULAR BIOLOGY, BIOPHYSICS a ...
325
829
Reporter Gene
Oct 11, 2008 02:12 AM
Discussion on 2D and 3D cell and tissue culture, primary cells, cell lines and transformed cells, isolation and culture of particular cell types such as stem cells, lymphocytes, epithelial and endothelial cells, transfection, electroporation, cell imaging, detachment, apoptosis…
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Jobs
This is a NEW Forum. Be 1st to post!
Cell Based Assay
Cell transfection, electroporation, cell imaging, microscopy, detachment, lysis, flow cytometry (FACS), analysis software, CellISA, fluorimetry, redox assays, apoptosis assays, proliferation / cell cycle assays.
This is a NEW Forum. Be 1st to post!
Cell/Tissue Culture
2D and 3D cell and tissue culture, primary cells, cell lines, transformed cells, cell isolation, media formulation, serum, antibiotics, suspension culture, division times, cell counting, viability assays
Unexplained cell death
Molecular Trafficking and Transport
Molecule transport in cells, endocytosis, pinocytosis, exocytosis, vesicles, membrane trafficking, nutrient receptors, lysosomes, proteosome, phagocytosis
This is a NEW Forum. Be 1st to post!
Stem Cell Technology
Human embryonic stem cells (hESCs), mESCs, differentiation assays, mouse embryonic fibroblast (MEF) feeder layers, feeder-free systems, pluripotency, adult (somatic) stem cells, embryoid bodies, surface markers, tertomas, polar bodies
This is a NEW Forum. Be 1st to post!
Transcription and Translation
Reporter Gene
Equipment
Use this forum to discuss incubators, laminar flow hoods, microscopes, cell counters, liquid nitrogen storage, cell storage, matrices, flasks, cytometers
This is a NEW Forum. Be 1st to post!
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
LacZ/Immunohistochemistry
Post Ideas/Find Collaborator
This is a NEW Forum. Be 1st to post!
Meeting Announcements
Molecular mechanisms of aging and age-related ...
173
394
Discussion on biochemical reactions inside the cell transmitting signals involving hormones, neurotransmitters, cytokines, growth factors, kinases, phosphotases, second messengers, transcription factors, protein and target genes…
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Jobs
Postdoctoral position at UCLA(US citizenship ...
Apoptosis
Cell death
detection of in vivo apoptosis
Cell Surface Receptors
Glycoproteins, plasma membrane, binding site, ligands, cytokines, hormones, growth factors, neurotransmitters, and adhesion molecules
chimera receptor construction help
GTPases, Kinases, Phasphatases, etc
Enzymes, signals, cell surface, downstream effects, cytoplasm
phospho p38 Immunohistochemistry
Kinases (RTKs)
Akt signalling in endosomes
Ras/Raf/ERK
MEK, cell proliferation, differentiation, and survival
Does K252a blocks MAPK activation?
STAT/JAK Pathways
Janus kinases (JAKs), tyrosine kinase activity, Signal Transducers and Activators of Transcription (STATs) SH2 domains, tyrosine-phosphorylated
jak/stat activation
TNF/TGF
Tumor necrosis factor-alpha(TNFα), cytokine, systemic inflammation, acute phase reaction, apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. Transforming growth factor beta (TGF-β), immunity, cancer, and heart disease.
Transient transfection
Equipment
good scanner?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Neutrophil Infiltration Assay
Post Ideas/Find Collaborator
Interesting article to read
Meeting Announcements
The American Society for Cell Biology Annual ...
89
167
Discussion of allele-specific mutation detection, diagnostic imaging, Pyrosequencing, Molecular Cytogenetics, Genetic Testing, Forensics, paternity testing. novel biomarkers, personalized medicine...
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Jobs
Biotech R&D Manager
Blood Test/Pathogens
determining PCR product of determined primers
Cancer Prognosis and Diagnosis
Current Screening and Testing, Non-invasive Methods, Early Detection, New Markers
Introgen files first gene therapy for FDA app ...
Diagnostics Imaging
Diagnostic imaging on iPhone
Diagnostic Sequencing
Sequenom Down syndrome test successful in stu ...
Histology
Fixation, embedding, staining, immunostaining and tissue diagnosis
mitochondria: images & videos
Paternity, Forensics
DNA Paternity Test Available Over-the-Counter
Tissue Typing/HLA
PCR SSP and PCR- SSO (principles)
Equipment
Lateral flow manufacturing
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
qPCR Newsletter July 2008 - main focus on qPC ...
Post Ideas/Find Collaborator
Molecular Diagnostics Take Center Stage
Meeting Announcements
Molecular Medicine Tri-conference 2008
122
248
Follow-on Biologics
Oct 05, 2008 02:30 AM
Discussion of drug discovery, including target validation, lead optimization, clinical trials, combinatorial chemistry
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Discover why 4 monochromators are better than ...
Jobs
Biotech Career Fair,
Animal Models
STZ induced rat model
Antibody/Protein Discovery
BSI: A breakthrough in molecular interaction ...
Biomarkers
Acidity as a tumor biomarker
Cancer & Small Drug Discovery
Biomarkers and image analysis tools
Discovery Informatics
Drug Abusing
Target Validation Techniques
Target Unrelated Phage
Therapeutic Proteins and Peptides
Therapeutic Sea Urchin Lectin that Kills Mala ...
Equipment
High Throughput Planar Patch clamp equipment ...
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Radioligand binding assays
Post Ideas/Find Collaborator
Follow-on Biologics
Meeting Announcements
2nd Annual Alzheimer’s Drug Development Summi ...
85
179
INTERPHEX 2009
Sep 15, 2008 13:28 PM
Discussion on pharmaceuticals from inception to the sales. Topics include assay development, upstream and downstream processing, scale up, and regulatory guidelines (both FDA and European)…
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Jobs
Director of Manufacturing: Corium Internation ...
Good Manufacturing Practice (GMP)
Control and management
Process Validation
Good Validation Practice (GVP)
Validation requirements
How are ranges of tablet press speeds handled ...
Regulatory Affairs (FDA and European Guidelines)
regulatory requirement for bioequivalence
Scale up, Isolation
Fermentation, cell culture, expression, and recovery
functional transferrin assay, TIBC
Upstream and Downstream Processing
Inception to recovery and purification
film forming polymer
Equipment
Theranostics equipments or test kits???
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
dendrimer purification
Post Ideas/Find Collaborator
US Department of Labor claims Drug manufactur ...
Meeting Announcements
INTERPHEX 2009
155
277
Reporter Gene
Oct 11, 2008 02:12 AM
Discussion of the broad topic of genetics, ranging from gene expression, gene therapy, and genetic engineering to transgenics…
Featured Products
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DNASTAR announces new Lasergene V8.0 Software ...
Jobs
Post Doctoral Fellow sought at Medical Colleg ...
Gene Delivery
Target cell, organs or whole animals
PEI PEGylation
Gene Discoveries/Expression
PCR and sequencing
Common disease/common variant hypothesis in q ...
Gene Therapy
One gene diseases, cell specific targeting, and new clinical trials
Continuing Down the Path of Gene Therapy
Genetic Engineering
Recombinant DNA, and gene splicing
Reporter Gene
Transgenics
Germ cells in fish to GFP expression in mice
Reporter Gene
Equipment
qPCR robots, sonoporators
Immunoblot with wrong band weights
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
MSX1 gene
Post Ideas/Find Collaborator
Genetics Based Jobs/ Salaries
Meeting Announcements
2008 Lupus Genetics Conference
168
400
Discussion on methodology for high-throughput genome scale analysis including next-generation sequencing, gene expression, SNP genotyping, CGH, bioinformatic approaches and useful software for genomic testing…
Featured Products
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DNASTAR announces new Lasergene V8.0 Software ...
Jobs
Tenure-Track Faculty Positions - Bioinformati ...
DNA Sequencing
Downstream analysis
Genome Analyzer II - Raw Data Analysis
Copy Number Variation
Interesting article
Functional Genomics
Wikipedia now has a stub for YFG (your favori ...
Gene Expression/Targeting
Microarrays and SAGE
Largest Linkage Scan to Date for Autism for M ...
Gene Expression
RT-PCR
The end for microarrays ?? - RNA Seq - ( sole ...
SNP Genotyping
High-throughput and genome scans
SNPlex
Translational Genomics
Clinic
SGC
Equipment
454 vs Solexa for high-throughput DNA sequenc ...
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
how to perform a Ka/Ks test
Post Ideas/Find Collaborator
Loads of genome-scale project ideas
Meeting Announcements
Whole Transcript expression meeting
357
816
do plants have immunity?
Oct 10, 2008 13:23 PM
Discussion of the Immune System including T cells, B cells, macrophages, Natural Killer T cells, bone marrow, chemokines, cytokines, innate immunity, blood types, immune response, inflammation…
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Jobs
Post Doctoral Fellow sought at Medical Colleg ...
Autoimmunity/Tolerance
Rheumatoid arthritis, type 1 diabetes, lupus, multiple sclerosis, inflammation, vitamin A, central tolerance, peripheral tolerance, APS-1, IPEX, auto antigens, immunnophenotyping, immunogenotyping
Anticardiolipin antibodies
Cancer Immunology
Cancer vaccines, tumor immunology, cancer antigens, tumor-specific antigens, cancer immunosurveillance, current clinical trials...
growth factor
Cellular and Molecular Immunology
CTL (cytotoxic T lymphocytes), macrophages, T cells, B cells, Helper T cells, Natural Killer Cells (NKT), complement, granulocytes, dendritic cells, antigen presentation, Memory B cells, Neutrophils, eosinophils, mast cells, innate immunity, phagocytosis, TH1, TH2, basophils, MHC, antigen presentation
CTL generation
Cytokines/Inflammation
The hematopoietins, interferons, TNF family members, common γ chain (γc), class II receptors, lymphokines, interleukins, FAS ligand (CD95L), adhesion, extravasation, rolling, chronic inflammation, delayed hypersensitivity, selectins, cytokine storms...
chimera receptor construction help
Immunity/transplantation
Antibodies, immunosuppression, organ transplantation, inoculation, vaccination, histocompatibility, adaptive immunity, antisera, antivenin...
do plants have immunity?
Immunochemistry
Complement regulatory molecules
Immunopathology
Pathological techniques to detect immune disorders
Anticomplement activity
Immunohistochemistry
NEED YOUR HELP~
Equipment
CAEV ELISA KITS
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Simultaneous measurement of dual-color IF and ...
Post Ideas/Find Collaborator
Why Viruses are More Virulent in Winter
Meeting Announcements
EMS India 2009 - First International Exhibiti ...
103
162
Discussion on novel techniques in automating laboratory techniques. Including microplate systems, liquid handling and dispensing systems, data management, robotics, total laboratory automation systems...
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Jobs
Medical Lab Technician in Automation Sought
HTS Proteomic Tools
shotgun proteomics
MicroArray/Biochip Technologies
Chip-chip linear amplification
HTS Equipment
96 well pipettor, microameters, and sequencers
Hand held 96-well pipetter?
Microplate Systems
Cell based assays, microelectronics and optimized instrumentation.
BMG LABTECHSs PHERAstar Plus sets new standar ...
Data Management
LIMS, Paperless Laboratory Systems
Web-Based LIMS: Right For Any Sized Lab
Robotics
Cell culture machines and integrative robotic platforms
Introducing Students to Lab robotics
Emerging Automated Technologies
Liquid Handling, dispensing, detection, separation and pre/post analytical automation
MatriCal provides automated cell culture.
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Semi-automated selection of DNA aptamers usin ...
General Discussion
qPCR NEWS March 2008 - qPCR robotics