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Scientist Solutions is a life sciences discussion board run by scientists for scientists with the mission to connect every scientist in the world. This site is moderated by a team of scientific staff. It is our hope that Scientist Solutions will encourage the exchange of information between investigative researchers and thereby contribute to the advancement of Science.
Below you will find discussion forums divided into basic life science categories. Post your question or insight in the appropriate category or use our special search tool to narrow your query. Before you leave don't forget to create your own profile in My Benchspace for networking with other scientists worldwide with whom you have interests and techniques in common. Together, we can problem solve, share and change the world.
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Online: 45   Welcome New Members: Luke F, Pedro Martins, Space Ace, marsha001, faehe, Bloodyj
Forum Topics Posts Last Post
528
1179
Rewards for participation
Aug 27, 2008 15:33 PM
Discussion of anything scientific that does not fit into another category or forum. Examples range from “how to write the statistical symbol for mean” to “my TC incubator is contaminated.”
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Jobs
Post Job Openings for General Here
Pharmaceutical Industrial Postdoctoral Scient ...
Announcements
Official announcements from the Scientist Solutions team.
Regarding Problem In Electronics
Comments and Suggestions
Request features or recommendations
private messages
Forum Suggestions
Recommend a new forum
Doing research in a foreign country
Meeting Announcements
CBI’s 4th Forum on Clinical Trial Registries ...
Student Questions
discuss academic related topics
graph
Miscellaneous
If you can't find the right place to post something, post it here!
Rewards for participation
214
328
John Locke
Aug 29, 2008 08:53 AM
Jokes
please keep it clean
A New Approach to Animal Testing
Quotes
Famous scientific quotes
Leverage
Anniversaries & Birthdays
famous scientists, major scientific achievements.
John Locke
Milestones & Dedications
Element 109
Strange Science Facts
DNA testing uncovers suspect sushi
Images & Movies
Stream in Iceland
191
445
Syngenta Job Postings
Aug 28, 2008 16:50 PM
Discussion chromatography (HPLC, FPLC, GC) spectroscopy, surface chemistry, thermal analysis, sample separation, extraction
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Ultrasound Equipment, Peptide Synthesizers, H ...
Meeting Announcements
Advances in Metabolomics Webinar presented by ...
Protocols
Library of policies and practices.
Glossary of HPLC/LC Separation Terms
Sample Preparation
Sample analysis problems
Sample Preparation Users Poll
Chromatography
help on chromatographic techniques.
chromatogram shape and interpretation - GFC
Methods
Chemistry methods and problems, rather than instrumental
Intracellular solution
Electron Spectroscopy/Microscopy
SEM, TEM, optical microscopy...
RTI International (www.rti.org) provides free ...
Electrophoresis (IEF, SDS-Page, 2D-Gel, Capillary, Native)
all electrophoretic techniques
Protein PEGylation
Laser Light Scattering
discuss light scattering techniques
Native protein molecular weight
Spectrometry (Atomic, Electron, Mass)
discuss spectroscopic methods
ICP-MS Recommendations for Pharmaceutical and ...
Surface Chemistry
surface analysis, etc
about Erbium
Equipment
information on purchasing and selling
Amersham Western blot
Post Ideas/Find Collaborator
Artificial skin model for teating of adhesion ...
Jobs
Analytical Chemistry openings
Syngenta Job Postings
207
627
BSA foam in patch solution
Aug 29, 2008 11:44 AM
Discuss organization of the body and its functions including organs, tissues, electrophysiology, histology, fixation, immunohistochemistry, exercise, muscle, microdissection…
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ASI PhotoTrack solves the problem of tracking ...
Jobs
Anatomy and Physiology Jobs
Funded Graduate Position in Manitoba (Canada)
Cell Anatomy/Physiology
Cellular morphology
call for images/video of mitochondria
Comparative Anatomy and Physiology
Organization of the body, organs and tissues
Joint load
Electrophysiology
Electrical properties cells/tissues.
BSA foam in patch solution
Human Anatomy/Physiology
Human organs/tissues/systems
Human Body being mostly water??
Equipment
Anatomical equipment
Santa Cruz c-fos antibody in brain tissue
Assay Development & Protocols
Protocols problems/design
elisa assay without a plate reader?
Post Ideas/Find Collaborator
Skeletal muscle sodium glucose co-transporter ...
Meeting Announcements
The World Congress on Controversies in Cardio ...
80
99
Discussion about breeding, diseases, genetics, management, ethics, nutrition and physiology of farm, laboratory, companion animals…
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SigmaPlot 10: The Simplest and Most Effective ...
Jobs
Animal and Veterinary Science Jobs
Assistant Professor / Veterinary Virologist
Anatomy and Physiology
internal and external structure and the physical relationships between the body’s constituents
images/video of mitochondria
Animal Behavior
Development, functions, and evolution animal behavior
Pheromone
Care and Ethics
How Can We Measure The Emotional States Of An ...
Animal Disease
Disease manifestation and treatments
Bovine Tuberculosis In Wildlife Threatens End ...
Ecology and Environmental Interactions
Relationships of animals to their environment.
Birds 'off the pace' with warming
Evolutionary Biology
Origin and descent of species
Introduction to Evolutionary Biology
Growth and Nutrition
Adding citrus juices or vitamin C to green te ...
Husbandry Technologies
breeding, feeding, housing and nutrition
Storage proteins
Laboratory Animals
Morpholinos for adult animals
Marine Biology
organisms in the ocean or brackish water.
Ocean Life Under Threat From Climate Change
Regulatory Issues
Regulatory Issues Surrounding the Use of Comp ...
Veterinary Science
medical, diagnostic, and therapeutics
Veterinary Pathology
Zoology & Developmental Zoology
Structure, physiology, development, and classification
Systems Biology: perturbing cellular gene reg ...
General Animal Science Discussion
Intradermal injection in mice
Meeting Announcements
2008 Joint Conference of AAZV/ARAV
212
541
scFv expression
Aug 26, 2008 15:16 PM
Discussion on antibodies techniques including polyclonal, monoclonal, peptide design, antisera, labeling, ELISA, fusion proteins, complement activation, isotypes, Fab, Fc, class switching…
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Jobs
Engineer - Automation/Instrumentation Job Ope ...
Antibody Fusion Proteins
soluble conjugates, blocking and activation reagents.
Ab / peptide immobilization
Epitope Mapping
immunogenic regions and antibody binding epitopes
T cell epitope mapping
Monoclonal Antibodies
clonal Abs
Help with Western Blot!
Recombinant Antibody Technology
Protein scaffolds for antibody mimics
Transgenic Animal Models
From TCR hybridomas to whole mouse genetic models...
transgenic mice/human antibodies
Equipment
ELISA plate readers, robots, flow cytometers, gel imagers
ELISA Plate Reader
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
scFv expression
Post Ideas/Find Collaborator
2007 Antibody Source Book from AbD Serotec
Meeting Announcements
Engineering the Next Generation of Antibodies
272
567
Discussion about chemical processes in living organisms. Including enzymes, kinetics, biosynthesis, catalysis, nutrient metabolism, glycolysis, amino acid synthesis…
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Monitor Biomolecular Reactions in Real Time
Jobs
Biochemistry Jobs
Experienced Biochemist
Chemical Carcinogenesis
chemical-induced mutagenesis, cancerous transformation of normal cells
Alcohol abuse affects millions
Chemiluminescence
luminescent detection assays
ECL detection limits
Enzymology
kinetics, cofactors, co-enzymes, metabolic pathways
Advances in Enzymology and Related Areas of M ...
Nuclear Magnetic Resonance (NMR)
quantum mechanical magnetic properties of an atom's nucleus.
'NMR On A Chip' Features NIST Magnetic Mini-s ...
Protein Structure/Modeling
three-dimensional modeling, de novo modeling, homology modeling
Improved Technique Determines Structure In Me ...
Equipment
What now?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Catalase assay
Post Ideas/Find Collaborator
substrate preparation in krabbe disease(conce ...
Meeting Announcements
DNA damage: from causes to cures
82
227
Discussion on issues in bioethics including medical bioethics, ethics in the laboratory, plagiary, euthanasia, cloning, artificial life, organ donation, stem cell use…
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GenomeLab SNPstream
General Bioethics
medical bioethics, ethics in the laboratory work space, fraud, plagairism
Are we teaching evolution to our children?
Jobs
Assistant/Associate Professor Position at Sto ...
Cloning/Stem Cell Therapy
ethical issues concerning human or animal stem cell therapy and cloning
Going overseas for experimental stem cell the ...
Conflicts of Interest
evolution, religion, biotechnology or intellectual property
Talking about God at the Clinic
Genetically Modified Plants
long-term effects of genetically modified plants as a food and fuel source
Genetically Modified Plants
Medical Gene Therapy
gene therapy in the wake of clinical failures
Medical Gene Therapy Could be the Wave of the ...
Post Ideas/Find Collaborator
life science networking
Bioethics
Meeting Announcements
American Society for Bioethics and Humanities ...
176
452
DNA Squencing Methods
Aug 26, 2008 14:09 PM
Discussion bioinformatics including software development, programming, systems biology, computational models, semantic web, ontologies, data extraction, gene annotation…
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DNASTAR signs Lasergene Multi-Site License Ag ...
Jobs
Tips for finding a suitable post-doc position
Miscellaneous
Software from a user perspective
Computational Biology Resources Lack Persiste ...
Systems Biology
Integrate different levels of information, wholistic rather than reductionist, computational models of biology.
ortholog detection by reciprocal BLAST
BiNS - Bioinformatics Student Network
ISCB Call for Applications
Semantic Web
Extension of the current web, data integration, ontology, knowledge management, web services...
wikiprofessional
Software Developers
How to start? (PDB Viewer)
Text and Data Mining
Extraction of information from unstructured, electronically accessible text resources
Curve pattern identification and categorizati ...
Workflows (Data Pipelines)
Workflows are series of programs that operate together to achieve goals such as the annotation of a genome. Discuss workflows that you've developed or that you would like to see built, and methods for building, running and sharing them (Perl, Taverna..)
Problem with web-Service for Taverna
Post Ideas/Find Collaborator
Need a collaborator? Read this first!
Meeting Announcements
International conference on applied bioengine ...
Sequence Analysis
DNA, Protein. Alignments (e.g. Blast), promoter identification, motif finding, transcription factor binding site prediction
DNA Squencing Methods
About this forum
What is your preferred journal to publish in, ...
44
89
Discussion on medical devices intended to interact with biological systems. Examples joint replacement, bone plates, cement, dental implants, heart valves, lens…
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Jobs
Biomedical Scientist opening
Biocompatibility
Ways to check for biocompatibility
Surface Modification
Material surface and biocompatibility
About C2C12
Biomaterials and Tissue Engineering
Hydrogels
Biomaterials and Medical Devices
Metals, biofilms, total hip replacement, artificial valves
Device to Manufacture Spider Silk
Biomaterials and Drug Delivery
Hiydrogels used for reparing human tissues
Meetings and Announcements
Tissue engineered bone
Post Ideas/Find Collaborators
Grant Ideas in Biomaterial Sciences
Assay Development & Protocols
This is a NEW Forum. Be 1st to post!
134
203
Interdisciplinary field applying techniques from the physical sciences to understanding biological structure and function at the molecular level.
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Pittcon Conference and Expo. New Orleans, Mar ...
Jobs
Biophysics Faculty
Crystallography/Crystallization
Arrangement of atoms in solids, uniform solution or melt
e-Crystallography course
Fluctuation Spectrometry
optical tweezer
Laser Confocal
High-resolution optical images, optical sectioning.
Nikon Instruments Launches Fully-Automated A1 ...
Raman Effect/FTIR
Killer Pulses Help Characterize Special Surfa ...
Scanning Probe Microscopy
Image surfaces at the nanometer scale
Nanotechnology: Femtogram-level Chemical Meas ...
Equipment
Electroporation cuvette gap size - Why?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Cell Biological Applications of Fluorescence ...
Post Ideas/Find Collaborator
Neutrinos: Tiny particles that fill the unive ...
Meeting Announcements
CELLULAR and MOLECULAR BIOLOGY, BIOPHYSICS a ...
486
1230
Docking of stem cell!!!!
Aug 28, 2008 15:34 PM
Discussion on 2D and 3D cell and tissue culture, primary cells, cell lines and transformed cells, isolation and culture of particular cell types such as stem cells, lymphocytes, epithelial and endothelial cells, transfection, electroporation, cell imaging, detachment, apoptosis…
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Jobs
Biomedical Scientist opening
Cell Based Assay
Cell transfection, electroporation, cell imaging, microscopy, detachment, lysis, flow cytometry (FACS), analysis software, CellISA, fluorimetry, redox assays, apoptosis assays, proliferation / cell cycle assays.
Separation of nuclei, cytosol and mitochondri ...
Cell/Tissue Culture
2D and 3D cell and tissue culture, primary cells, cell lines, transformed cells, cell isolation, media formulation, serum, antibiotics, suspension culture, division times, cell counting, viability assays
CV1 transfection using Lipofectamine 2000
Molecular Trafficking and Transport
Molecule transport in cells, endocytosis, pinocytosis, exocytosis, vesicles, membrane trafficking, nutrient receptors, lysosomes, proteosome, phagocytosis
proteasome storage granules
Stem Cell Technology
Human embryonic stem cells (hESCs), mESCs, differentiation assays, mouse embryonic fibroblast (MEF) feeder layers, feeder-free systems, pluripotency, adult (somatic) stem cells, embryoid bodies, surface markers, tertomas, polar bodies
Docking of stem cell!!!!
Transcription and Translation
ChIP contamination?
Equipment
Use this forum to discuss incubators, laminar flow hoods, microscopes, cell counters, liquid nitrogen storage, cell storage, matrices, flasks, cytometers
Smallest volume electroporation device
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
MTT assay
Post Ideas/Find Collaborator
Who's looking @ hERG (Ether-a-go-go Related G ...
Meeting Announcements
Molecular mechanisms of aging and age-related ...
179
393
detection of in vivo apoptosis
Aug 29, 2008 12:06 PM
Discussion on biochemical reactions inside the cell transmitting signals involving hormones, neurotransmitters, cytokines, growth factors, kinases, phosphotases, second messengers, transcription factors, protein and target genes…
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Jobs
Postdoctoral Scientist position at Harvard
Apoptosis
Cell death
detection of in vivo apoptosis
Cell Surface Receptors
Glycoproteins, plasma membrane, binding site, ligands, cytokines, hormones, growth factors, neurotransmitters, and adhesion molecules
GTPgammaS Binding Assay
GTPases, Kinases, Phasphatases, etc
Enzymes, signals, cell surface, downstream effects, cytoplasm
phospho p38 Immunohistochemistry
Kinases (RTKs)
Akt signalling in endosomes
Ras/Raf/ERK
MEK, cell proliferation, differentiation, and survival
ras/raf/erk
STAT/JAK Pathways
Janus kinases (JAKs), tyrosine kinase activity, Signal Transducers and Activators of Transcription (STATs) SH2 domains, tyrosine-phosphorylated
jak/stat activation
TNF/TGF
Tumor necrosis factor-alpha(TNFα), cytokine, systemic inflammation, acute phase reaction, apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. Transforming growth factor beta (TGF-β), immunity, cancer, and heart disease.
Transient transfection
Equipment
good scanner?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Neutrophil Infiltration Assay
Post Ideas/Find Collaborator
Interesting article to read
Meeting Announcements
The American Society for Cell Biology Annual ...
89
163
Diagnostic imaging on iPhone
Aug 29, 2008 03:38 AM
Discussion of allele-specific mutation detection, diagnostic imaging, Pyrosequencing, Molecular Cytogenetics, Genetic Testing, Forensics, paternity testing. novel biomarkers, personalized medicine...
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The Vaccines Development Forum
Jobs
Biotech R&D Manager
Blood Test/Pathogens
determining PCR product of determined primers
Cancer Prognosis and Diagnosis
Current Screening and Testing, Non-invasive Methods, Early Detection, New Markers
Introgen files first gene therapy for FDA app ...
Diagnostics Imaging
Diagnostic imaging on iPhone
Diagnostic Sequencing
Sequenom Down syndrome test successful in stu ...
Histology
Fixation, embedding, staining, immunostaining and tissue diagnosis
mitochondria: images & videos
Paternity, Forensics
DNA Paternity Test Available Over-the-Counter
Tissue Typing/HLA
PCR- SSP in comparison to PCR- SSOP
Equipment
Lateral flow manufacturing
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
qPCR Newsletter July 2008 - main focus on qPC ...
Post Ideas/Find Collaborator
BioIQ
Meeting Announcements
Molecular Medicine Tri-conference 2008
139
262
It's a risky business
Aug 29, 2008 01:44 AM
Discussion of drug discovery, including target validation, lead optimization, clinical trials, combinatorial chemistry
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Get high quality protein mass spec data from ...
Jobs
Biotech Career Fair,
Animal Models
STZ and ZDF rats
Antibody/Protein Discovery
BSI: A breakthrough in molecular interaction ...
Biomarkers
Acidity as a tumor biomarker
Cancer & Small Drug Discovery
Biomarkers and image analysis tools
Discovery Informatics
Drug Abusing
Target Validation Techniques
Target Unrelated Phage
Therapeutic Proteins and Peptides
Therapeutic Sea Urchin Lectin that Kills Mala ...
Equipment
High Throughput Planar Patch clamp equipment ...
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Radioligand binding assays
Post Ideas/Find Collaborator
It's a risky business
Meeting Announcements
Advances in Metabolomics Webinar presented by ...
86
179
Discussion on pharmaceuticals from inception to the sales. Topics include assay development, upstream and downstream processing, scale up, and regulatory guidelines (both FDA and European)…
Featured Products
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Biodot Workshop: Emerging Technologies to Tra ...
Jobs
Director of Manufacturing: Corium Internation ...
Good Manufacturing Practice (GMP)
Control and management
Process Validation
Good Validation Practice (GVP)
Validation requirements
How are ranges of tablet press speeds handled ...
Regulatory Affairs (FDA and European Guidelines)
regulatory requirement for bioequivalence
Scale up, Isolation
Fermentation, cell culture, expression, and recovery
functional transferrin assay, TIBC
Upstream and Downstream Processing
Inception to recovery and purification
film forming polymer
Equipment
Theranostics equipments or test kits???
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
dendrimer purification
Post Ideas/Find Collaborator
US Department of Labor claims Drug manufactur ...
Meeting Announcements
4th Annual Global Pharma Manufacturing Summit
159
280
Discussion of the broad topic of genetics, ranging from gene expression, gene therapy, and genetic engineering to transgenics…
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The New and Improved RunOne Electrophoresis S ...
Jobs
Genetic Research Director sought in Philadelp ...
Gene Delivery
Target cell, organs or whole animals
Autism Spectrum Disorder - Contribution of SH ...
Gene Discoveries/Expression
PCR and sequencing
Curated list of tumor tupressor genes?
Gene Therapy
One gene diseases, cell specific targeting, and new clinical trials
Continuing Down the Path of Gene Therapy
Genetic Engineering
Recombinant DNA, and gene splicing
President Bush signs Genetic Information Non ...
Transgenics
Germ cells in fish to GFP expression in mice
Nude and SCID
Equipment
qPCR robots, sonoporators
Immunoblot with wrong band weights
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
MSX1 gene
Post Ideas/Find Collaborator
PCR HELP!!
Meeting Announcements
American Society of Human Genetics Meeting (P ...
194
406
Discussion on methodology for high-throughput genome scale analysis including next-generation sequencing, gene expression, SNP genotyping, CGH, bioinformatic approaches and useful software for genomic testing…
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New Packaged Applications for Beckman Coulter ...
Jobs
Job postings at the BC Genome Sciences Centre ...
DNA Sequencing
Downstream analysis
who has the protocol of mRNA 3' end library c ...
Copy Number Variation
Interesting article
Functional Genomics
Wikipedia now has a stub for YFG (your favori ...
Gene Expression/Targeting
Microarrays and SAGE
Largest Linkage Scan to Date for Autism for M ...
Gene Expression
RT-PCR
The end for microarrays ?? - RNA Seq - ( sole ...
SNP Genotyping
High-throughput and genome scans
High throughput genotyping
Translational Genomics
Clinic
SGC
Equipment
454 vs Solexa for high-throughput DNA sequenc ...
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
how to perform a Ka/Ks test
Post Ideas/Find Collaborator
Loads of genome-scale project ideas
Meeting Announcements
SNP vs CNV webinar at Science.com
101
212
Chip-chip linear amplification
Aug 10, 2008 13:56 PM
Discussion on flow cytometry, genome analysis, proteomic tools, microarrays, algorithms…
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High-Quality Custom Oligo Synthesis
Jobs
Assistant Biologist
Algorithms for Managing and Inferring Pathways
Sequence of instructions, calculation, statistical analysis biopathways
selective algorithms
Flow Cytometry
Data acquisition, limited sample volume, and multi-sample data sets
Linearization on Fluorescene signals
Genome Analysis
Microchips and microarray
microchip for genome sequencing
High-Throughput Proteomic Tools
Gel free mass spectrometry
shotgun proteomics
Microarray/ Biochip Technologies
Chip-chip linear amplification
Equipment
96 well pipettor, microameters, and sequencers
Hand held 96-well pipetter?
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Protocol for 2 color microarray labeling and ...
Post Ideas/Find Collaborator
Ask a question or share a comment with the HTS field
HTS Portal
Meeting Announcements
Web Seminar May 29th: A high-throughput scree ...
354
774
Two colour acquisition problem
Aug 29, 2008 16:26 PM
Discussion of the Immune System including T cells, B cells, macrophages, Natural Killer T cells, bone marrow, chemokines, cytokines, innate immunity, blood types, immune response, inflammation…
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9th Annual - Immunogenicity for Biotherapeuti ...
Jobs
inflammation and cancer
Autoimmunity/Tolerance
Rheumatoid arthritis, type 1 diabetes, lupus, multiple sclerosis, inflammation, vitamin A, central tolerance, peripheral tolerance, APS-1, IPEX, auto antigens, immunnophenotyping, immunogenotyping
Anticardiolipin antibodies
Cancer Immunology
Cancer vaccines, tumor immunology, cancer antigens, tumor-specific antigens, cancer immunosurveillance, current clinical trials...
growth factor
Cellular and Molecular Immunology
CTL (cytotoxic T lymphocytes), macrophages, T cells, B cells, Helper T cells, Natural Killer Cells (NKT), complement, granulocytes, dendritic cells, antigen presentation, Memory B cells, Neutrophils, eosinophils, mast cells, innate immunity, phagocytosis, TH1, TH2, basophils, MHC, antigen presentation
Dendritic Cell Antigen Presentation
Cytokines/Inflammation
The hematopoietins, interferons, TNF family members, common γ chain (γc), class II receptors, lymphokines, interleukins, FAS ligand (CD95L), adhesion, extravasation, rolling, chronic inflammation, delayed hypersensitivity, selectins, cytokine storms...
Xenotransplantation - graft rejection detecti ...
Immunity/transplantation
Antibodies, immunosuppression, organ transplantation, inoculation, vaccination, histocompatibility, adaptive immunity, antisera, antivenin...
must see
Immunochemistry
mouse on mouse,what should I do?
Immunopathology
Pathological techniques to detect immune disorders
Anticomplement activity
Immunohistochemistry
NEED YOUR HELP~
Equipment
CAEV ELISA KITS
Assay Development & Protocols
Also, remember to visit the protocols tab on the homepage toolbar
Two colour acquisition problem
Post Ideas/Find Collaborator
Why Viruses are More Virulent in Winter
Meeting Announcements
Allergy, Asthma and Immunology Update