Research

Research in our lab is focused on the molecular mechanisms underlying neuronal cell fate specification and differentiation. Specifically, we are studying how soluble signaling molecules, such as Sonic hedgehog, retinoic acid and Wnts, positively and negatively regulate cell cycle progression, gene expression and process outgrowth/migration, thereby promoting neuronal differentiation from multipotent progenitor cells in the developing nervous system. We are also testing whether the signaling molecules known to play essential roles in neural development can promote differentiation and survival of adult stem cells. Using phenotypically defined pluripotent cell populations found in various adult tissues (bone marrow, adipose and muscle), we are testing whether these adult stem cells can give rise to functional neurons if appropriate signals are provided at appropriate timing. To address these questions, we employ a range of research approaches, including molecular/cell biology, biochemistry, genetics and embryology.

Cell / Tissue Culture, Centrifugation, Cloning, DNA Isolation & Purification, PCR / RT-PCR, Plasmid Isolation & Purification, Protein-Nucleic Acid Interaction Analysis, Real Time PCR / RT-PCR, RNA Isolation & Purification, RNAi & Gene Silencing Assays, Transfection

Centrifuge, Confocal Microscope, Imaging Systems, Incubators, Multi-Channel Pipettors, Orbital Shakers, PCR Kits, PCR Thermocycler, Pipettors, Real Time PCR Thermocycler, RNA Interference / Gene Silencing Kits, Vacuum Equipment, Vortexing Equipment, Water Purification Systems

Neuroscience

www.invitrogen.com, www.fishersci.com, www.sigma-aldrich.com, www.iupui.edu

- Triathlons

- Football

- Organic Gardening