Protocol for: Cyclin D1 Reporters
Abstract or Description:
Regulated progression through the cell cycle requires sequential expression of a family of proteins called cyclins. Frank McCormick's group at UCSF recently deposited a series of human cyclin D1 promoter constructs from his Nature 1999 publication with Addgene. Cyclin D1 is over-expressed in many colon carcinomas and has been identified as a target of β-catenin mediated transcription via the core TCF/LEF-binding consensus sites in the promoter region. The McCormick group demonstrates this regulation using Cyclin D1 luciferase reporter constructs containing mutated TCF-binding sites.
To request related plasmids contact Addgene, the non-profit plasmid repository
Citation: Tetsu O, McCormick F. Nature 1999 Apr 1;398(6726):422-6
Posted By: jkamens
on 5/9/2012 1:41:40 PM