Protocol for

Localization of Subcutaneous, Intraperitoneal, and Satellite Tumors

Link to Full Protocol: http://www.carestreamhealth.com/localization-ard-in-multi-AP ...

Abstract or Description:

Non-invasive in vivo optical imaging has been increasingly used in the pre-clinical arena. Tumorigenesis studies, in particular, have lent themselves to fluorescent tracking as a means of tumor detection, growth monitoring, and determining the efficacy of treatment(s). Monitoring the growth and early detection of tumors at both the primary and secondary sites is of principal interest to the researcher. Light-scattering, narrow visualization windows in deep tissue, and inability of fluorescent signal to penetrate completely through animals further compounds the difficulties of visualization and analysis. As a result, detection of signal often requires the animal to be positioned at an optimal orientation - one in which the origin of the fluorophore is positioned so that the light path through diffusive tissue is minimized. Further, in many studies tumors originate at unanticipated locations, making it difficult to properly position the mouse for optimal detection of tumors. To overcome these issues, we have developed a murine rotation device for use in the commercially available Carestream In Vivo Imaging Systems that enables visualization of the mouse at multiple calibrated angles. Application of this novel animal rotation device demonstrated its utility for the detection and longitudinal monitoring of subcutaneous, intraperitoneal, and secondary tumor sites non-invasively in live mice.

Citation:

Attachments: No Attachments

Posted By: Carestream Health on 1/28/2011 11:04:46 AM

Comments

Add Comment