Dualsystems Total protein extraction kit (Yeast/Fungi)

 Protein extraction from fungi and bacteria can be challenging, due to the stable cell wall which must be disrupted prior to protein extraction and solubilization. Most often, mechanical disruption methods, such as French Press or glass beads are used to remove the cell wall, followed by detergent based extraction of total protein.

 The total protein extraction kit has been specially formulated for efficient extraction and solubilization of total protein mixtures from fungi and bacteria. After mechanical disruption of the cell wall by glass beads, proteins are solubilized using a complex detergent mix. The use of several detergents ensures uniform solubilization of proteins with widely different characteristics, including strongly hydrophobic proteins, such as integral membrane proteins. After solubilization, proteins are denatured by addition of sample buffer and loaded onto an SDS- PAGE gel for further analysis