Protocol for: Labeling and In Vivo Localization of Ovarian Cancer Cells
Abstract or Description:
Ovarian cancer is the fourth-leading cause of cancer-related deaths and the leading cause of gynecological cancer deaths. Recurrence and chemoresistance are the major hurdles in the management of patients with ovarian cancer. Ovarian cancer stem cells (OvCSC) have been postulated as the potential source of recurrence and chemoresistance. Persistence of chemoresistant OvCSCs after debulking or chemotherapy may “heal” the cancer and lead to recurrence. Therefore, identification of OvCSCs and their complete removal is a pivotal stage for the treatment of ovarian cancer. Recent advances in reagent chemistry have facilitated the development of near-infra red (NIR) emitting probes ideal for optical localization of tumor cells in vivo. The objective of the following study was to develop a new in vivo imaging model that allows for the detection and monitoring of OvCSCs.