Protocol for: Collagen type I Coating Procedures
Abstract or Description:
Start by using collagen
as a thin coating at 5-10 ug per cm2
, but you may need to optimize these values depending on your application.
1) Dilute material to 50 µg/ml using 0.01M HCl.
2Add enough diluted material to coat dishes with 5-10 µg/cm2.
A 35mm dish has a surface area of approximately 10 cm2. One to two milliliters of the above solution would be
sufficient to cover the dish.
4) Carefully aspirate remaining solution.
may be used immediately or air dried. They may then be stored at 2-8o
C for up to one week under
Posted By: TheFFM
on 7/14/2010 1:27:24 PM