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Collagen type I Coating Procedures

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Abstract or Description:


Start by using collagen as a thin coating at 5-10 ug per cm2, but you may need to optimize these values depending on your application.

1) Dilute material to 50 µg/ml using 0.01M HCl.
 
2Add enough diluted material to coat dishes with 5-10 µg/cm2.
For example:
 
A 35mm dish has a surface area of approximately 10 cm2. One to two milliliters of the above solution would be
sufficient to cover the dish.
 
3) Incubate at room temperature for one hour.
4) Carefully aspirate remaining solution.
5) Rinse well to remove acid, using PBS or serum free medium.
6) Plates may be used immediately or air dried. They may then be stored at 2-8oC for up to one week under
sterile conditions.

Citation:
Attachments: No Attachments
Posted By: TheFFM on 7/14/2010 1:27:24 PM
Comments
 
1.
Rajeshwari patel (Sep 22, 2010, 0:58 AM)
IF unqual amount of collagen is remai in the plates what should be the precusion? And it is same for flask Also?
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