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Immunostaining protocols for flow cytometric analysis of adherent cells

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Abstract or Description:
 Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population. This technique is most commonly used with non-adherent cells, but it can also be an effective tool for analyzing protein expression in adherent cells. The following immunostaining protocol outlines the steps required for detecting surface or intracellular antigens in adherent cells by flow cytometry. Data is presented to show the staining of SSEA-4 (surface) and Oct-3/4 (intracellular) in BG01V embryonic stem cells using this protocol.



Citation: DOI: 10.2144/000113280
Attachments: No Attachments
Posted By: TheFFM on 5/21/2010 1:39:05 PM
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