The eosinophil count is performed by diluting whole blood with a staining solution. The phyloxine B present in the diluting fluid stains only the eosinophils red; all other leukocytes are preserved but not stained.1,2,3 The diluted specimen is charged onto a hemacytometer for counting. Using the low-power (10x) objective, the eosinophils appear bright orange-red and are clearly distinguishable from neutrophils, basophils, lymphocytes, and monocytes, which do not stain. http://wps.prenhall.com/wps/media/objects/684/700987/ch07EOSI.htm
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