Protocol for: ND96 Solution for electrophysiology recordings from Xenopus oocytes
Abstract or Description:
Fw Mass in 1 litre
93.5 mM NaCl 58.44 5.464 g
2mM KCl 74.55 149 .1 mg
1.8 mM CaCl2.2H2O 147.01 264.6 mg
2 mM MgCl2(.6H2O) 203.3 406.6 mg
5 mM HEPES 238.3 1.192 g
Adjust to pH 7.5 with NaOH (contributing an additional 2.5 mM Na to the final solution)
Osmolarity should be 195 ± 5 mOsm
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Posted By: TheFFM on 3/11/2010 2:53:11 PM