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ND96 Solution for electrophysiology recordings from Xenopus oocytes

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Abstract or Description:

                                                                  Fw                 Mass in 1 litre                      

93.5 mM NaCl                                      58.44              5.464 g

2mM KCl                                               74.55              149 .1 mg

1.8 mM CaCl2.2H2O                          147.01            264.6 mg

2 mM MgCl2(.6H2O)                            203.3              406.6 mg

5 mM HEPES                                        238.3             1.192 g

 

Adjust to pH 7.5 with NaOH (contributing an additional 2.5 mM Na to the final solution)

Osmolarity should be 195 ± 5 mOsm

Citation:
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Posted By: TheFFM on 3/11/2010 2:53:11 PM
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1.
TheFFM (Mar 11, 2010, 17:26 PM)
Variations include using these substitutes 1.8 mM CaCl2 (anhydrous) 199.8 mg/L OR 1.8ml/L 1M CaCl2 Stock solution 2ml/L 1M MgCl2 stock solution 667 ul/L of 3M KCL stock solution
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