Scientist Solutions: International Life Science Community By Scientists For Scientists
Home » Protocols » Maintaining Lymphoblastoid Cell Lines
Thanks to our sponsors who make this site possible
Protocol for

Maintaining Lymphoblastoid Cell Lines

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Abstract or Description:

This protocol is explains about growth of  lymphoblastoid cells for permanent storage and for DNA extraction.

Safety Considerations:

      All cultured animal and human cells have the potential for carrying viruses, latent viral genomes, and other infectious agents. Cell cultures should be handled very carefully by trained persons under laboratory conditions which afford adequate biohazard containment. A Biological Safety Cabinet must be used when passaging cell lines. Use bleach in a suctioning apparatus to kill unused virus. All material used in passaging the cell lines must be autoclaved. Gloves are always worn to protect hands from contamination . A laboratory coat should be worn to protect clothes from contamination. Doors of the tissure culture room should remain closed to decrease the amount of airborn contaminants entering the incubators and the room. Equipment (incubators, centrifuges, microscopes, tabletops, etc.) should be cleaned routinely to help maintain a sterile work environment.

Attachments: No Attachments
Posted By: muthuonco on 11/25/2009 6:44:41 AM
Add Comment