Scientist Solutions: International Life Science Community By Scientists For Scientists
Home » Protocols » Minimum inhibitory concentration (MIC)
Thanks to our sponsors who make this site possible
Protocol for

Minimum inhibitory concentration (MIC)

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Abstract or Description:

Minimum Inhibitory Concentration

1.    Grow cultures up overnight.
2.    The following day, inoculate a fresh culture with a 1:10-20 dilution and grow up to OD600 0.4.
3.    Dilute to OD600 0.0005 (1:800) in THB (or relevant media).
4.    Dilute this 1:200 and drop about 25µl on THA (or relevant agar plates) to confirm equal starting inoculums.
5.    Put 50µl of the 0.0005 cultures in a 96 well plate in triplicates.
6.    Add 50µl per well of the substance you want to test (ie. antimicrobial peptide, antibiotic) to each well, keeping in mind the final concentration will be half the original concentration you are adding. It is good to test 1:2 dilutions (in THB, or relevant media) of this substance.
7.    Incubate overnight and check by eye or OD600 24 hours later to determine what concentration inhibited the growth of the different strains of bacteria.


Attachments: No Attachments
Posted By: A Timmer on 1/12/2009 6:26:42 PM
Add Comment