Scientist Solutions: International Life Science Community By Scientists For Scientists
Home » Protocols » Lymphocyte proliferation using succinimidyl ester of carboxyfliorescein diacetate
Thanks to our sponsors who make this site possible
Protocol for

Lymphocyte proliferation using succinimidyl ester of carboxyfliorescein diacetate

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Abstract or Description:

Lymphocyte proliferation using succinimidyl ester of carboxyfliorescein diacetate


The succinimidyl ester of carboxyfluorsecein diacetate [5(6)]- CFSE is the best reagent currently available for the analysis of cellular proliferation. CFSE spontaneously and irreversibly couples to both intracellular and cell surface proteins by reaction with lysine side chains and other available amine groups. When cells divide, CFSE labeling is distributed equally between the daughter cells, which are therefore half as fluorescent as the parents. As a result, halving of cellular fluorescence intensity marks each successive generation in a population of proliferating cells and is readily followed by flow cytometry. The number of divisions, which can be followed, is limited only by the auto fluorescence level of unlabeled cells, and the uniformity in size of the labeled cell population (Hodgkin et al., 1996).

Attachments: No Attachments
Posted By: Nils Holgerson on 1/16/2007 12:00:00 AM
Add Comment
Click here to
Become a member & join our
community (It's easy & free)
Already a member? Please log in
User Name  
Forget Password?
Post a new protocol

You must be logged in to post. Log in above.
Not a member yet? Click here to register
(it's free)
Thank You to Our Sponsor