Protocol for: Measurement of Mouse and Human Interferon ?
Abstract or Description:
This unit describes two protocols that can be used to quantitate interferon (IFN-), an important modulatory lymphokine that regulates natural, cell
-mediated, and humoral immunity
by eliciting a number of biological responses in many different cell
types. Depending upon the specificity of the reagents used, the assays
will detect either human or murine IFN-. The Basic Protocol is an enzyme
-linked immunosorbent assay
) based on IFN-'s unique antigenic structure. The Alternate Protocol is a functional activity assay
based on IFN-'s ability to induce major histocompatibility (MHC) class II antigens (also termed Ia antigens) on responsive cells
. The advantage of the ELISA
protocol is its specificity, while the advantage of the MHC protocol is its sensitivity.
Citation: DOI: 10.1002/0471142735.im0614s18
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