Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the
My Lab Drawer section of your bench space.
Available to members only. Please log in or
register for your free account now.
EMBL Introductory Course Basic Light Microscopy
Jul 10, 2012 - Jul 13, 2012
Event Type: Conference
Location: EMBL Heidelberg, Germany
Registration Deadline: May 13, 2012
Abstract Submission Deadline: None
Description of event:
EMBL Introductory Course
EMBL Heidelberg, Germany Tuesday 10 July - Friday 13 July 2012
Summary
Light microscopy is the most widely used form of
microscopy for organic and inorganic research. The correct handling of the
microscope and optimizing
image quality (e.g. contrast,
resolution) according to different applications are crucial to obtaining the best results out of a
light microscope.
This four-day course will provide the participants with an overview about the basics of
light microscopy and
imaging. The course will combine lectures with daily hands-on practices followed by discussion. The participants will get familiarized with the setup and potential of
light and
fluorescence microscopes and they will be given an insight of the basics of
digital imaging, including
image processing.
Who Should Apply
This course is directed towards PhD students and faculty searching for instruction on
light microscopy and
image processing.
Topics
Topics to be covered:
Basics of optics and microscopy
• Theoretical basics of light and optics
• Types of microscopes frames – and types of optics
• Set up bright field microscopes and the appropriate optics for different needs.
• Cleaning, centering adjusting and operating a microscope
• Basic set up and use of imaging devices
Contrast methods
• Introduction into contrast methods (phase contrast and differential interference contrast/DIC)
Contrast methods - set up and alignment
• Set up and use of contrast methods: phase contrast and differential interference contrast/DIC
• Oblique Illumination or Hoffmann Modulation Contrast - an alternative to DIC?
Basics of digital imaging
• Adaptation of digital cameras and their alignment
• Interpretation of image content, histogram and digital filter functions
• Improving digital image contrast
• Discussing open questions
Contact
EMBL Course and Conference Office
European Molecular Biology Laboratory
Meyerhofstraße 1
69117 Heidelberg
Germany