Event Type: Conference
Location: EMBL Heidelberg, Germany
Registration Deadline: Sep 20, 2012
Abstract Submission Deadline: None

Description of event:

Zinc finger nucleases (ZFNs) enable manipulation of the genome with unprecedented ease and precision. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) in DNA at user-specified locations. DSBs are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination and non-homologous end joining.


Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (knockouts), integrations, or modifications.


Featured lectures will provide in-depth discussion on the fundamentals of ZFNs as well as cutting edge applications for targeted gene knockout, integration and tagging. Additionally, each of the participants will conduct laboratory exercises to become proficient in various techniques for ZFN delivery, identification of mutants, and methods for quantitating ZFN efficiency. Registrants should be familiar with basic mammalian cell culture techniques, PCR and cloning.

Local Transport

Participants staying at the BoardingHouse Heidelberg: Shuttle buses will go from the hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available before the meeting.

Participants staying in other hotels in the centre of Heidelberg: Transport from other hotels is not provided and participants will need to find their own way to EMBL.
You can of course use the shuttle service from to the BoardingHouse Heidelberg bus stop.

For further information follow the link  Public Transportation to the Venue
Course Venue
   

EMBL Advanced Training Centre
European Molecular Biology Laboratory
Room: ATC Flex Labs A + B, Training Lab B
Meyerhofstraße 1
69117 Heidelberg, Germany

EMBL Course and Conference Office
European Molecular Biology Laboratory
Meyerhofstraße 1
69117 Heidelberg
Germany

Conference Website:
http://www.embl.de/training/events/2012/ZFN12-02/index.html