Protocol for Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues
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Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages in three-dimensional and deep tissue imaging. This unit will describe the basic physical principles of two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate localized photochemistry.
Citation: DOI: 10.1002/0471142301.ns0814s17
Attachments: No Attachments
Protocol for: Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues Abstract or Description: Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages in three-dimensional and deep tissue imaging. This unit will describe the basic physical principles of two-photon excitation and discuss the advantages and limitations of its use in laser- scanning microscopy. The advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate localized photochemistry. Citation: DOI: 10.1002/0471142301.ns0814s17 Attachments: No Attachments
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