http://www.scientistsolutions.com/ Latest discussion from ScientistSolutions.com Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t13893-hematopoietic+stem+cell+transplantation.html Mon, 08 Feb 2010 17:31:55 GMT http://www.scientistsolutions.com/t13892-instrument_independent+lc%2c+gc+_amp%3b+ms+training+courses+europe+2010+_amp%3b+2011.html Visit www.lc-gc-ms-instrument-training-courses.com for further details.]]> Mon, 08 Feb 2010 13:59:34 GMT http://www.scientistsolutions.com/t13891-instrument_independent+lc%2c+gc+_amp%3b+ms+training+courses+europe+2010+_amp%3b+2011.html Visit www.lc-gc-ms-instrument-training-courses.com for further details.]]> Mon, 08 Feb 2010 13:54:39 GMT http://www.scientistsolutions.com/t13890-plasmid.html pls help me with explanation..]]> Mon, 08 Feb 2010 13:46:01 GMT http://www.scientistsolutions.com/t13889-instrument_independent+lc%2c+gc+_amp%3b+ms+training+courses+europe.html Visit www.lc-gc-ms-instrument-training-courses.com for further details.]]> Mon, 08 Feb 2010 13:39:30 GMT http://www.scientistsolutions.com/t13888-instrument_independent+lc%2c+gc+_amp%3b+ms+training+courses+across+europe.html If there are any analysts out there searching for reasonably priced GC/GC-MS, headspace analysis or LC/LC-MS training courses in Europe, you may be interested to learn of a new series of instrument-independent courses on offer in 2010-2011. Training courses designed to teach you how to use any instrument model - without the sales pitch (they dont sell products)!
Visit www.lc-gc-ms-instrument-training-courses.com for further details.]]> Mon, 08 Feb 2010 13:37:53 GMT http://www.scientistsolutions.com/t13887-lab+bratz+episode+%23196.html Mon, 08 Feb 2010 06:48:32 GMT http://www.scientistsolutions.com/t13886-protein+isolation+from+tissue.html I am trying to isolate protein from human pancreatic tissue. I need to isolate cytoplasmic and nuclear proteins seperately. Can you please help me]]> Mon, 08 Feb 2010 03:43:42 GMT http://www.scientistsolutions.com/t13885-why+are+stem+cells+shiny_.html Someone hypothesized its something to do with the high nucleus:cytoplasm size ratio, but I don't see how this could work.
Thanks!]]> Sun, 07 Feb 2010 22:13:11 GMT http://www.scientistsolutions.com/t13884-iron+analysis.html I just wanted to start creating some topics which most of us in the environmental field deal with on a pretty regular basis. This way we can see how other scientists deal with some of these problems as can those scientists and chemists new to the environmental field get a good idea for how they can handle such an issue as interferences in Iron analysis.
Iron! This is an element which many of us are running on ICP-MS. It is also an element which is subject to ...]]> Sun, 07 Feb 2010 16:00:15 GMT http://www.scientistsolutions.com/t13883-co_ip+problem+regarding+negative+control.html I met a strange problem regarding negative control
I transfect HEK293 T cells with experiemnt group : A+B plamids; control group: A plasmid only. 48 hrs after transfection, I harvest cells, using mouse Anti-B antibody to pull down protein B, then detect protein A with mouse anti-A Ab. Finally, both goups show strong bands.
When I use anti-A antibody to pull down protein A, then detect protein B, the result is very good.
Initially, I though...]]> Sun, 07 Feb 2010 09:01:06 GMT http://www.scientistsolutions.com/t13882-peak+split.html Normal
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{mso-...]]> Sun, 07 Feb 2010 02:58:30 GMT http://www.scientistsolutions.com/t13881-pcr+_amp%3b+nested+pcr+problems.html I performed the first round PCR for PCV2. Positive and negative controls were included. Except for the positive control, all samples and negative control were negative by the 1st round PCR. Using this pcr product as the template, nested PCR was performed. Utmost care was taken to avoid any contamination by using one pipettes tips per sample although same pipettes were used for the pcr and nested pcr. My lab do...]]> Sun, 07 Feb 2010 01:51:14 GMT http://www.scientistsolutions.com/t13880-lab+bratz+episode+%23195.html Sat, 06 Feb 2010 08:54:06 GMT http://www.scientistsolutions.com/t13879-how+to+calculate+the+equilibrium+constant+and+rate+constant+of+backward+rxn.html In a first order reversible reaction, at a temperature T, the standard molar free energy is equal to -2.303 RT , the rate constant of forward reaction is equal to 1x10^-3 s^-1. How to calculate equilibrium constant and rate constant of backward rxn,,?
help me pls..]]> Sat, 06 Feb 2010 07:38:20 GMT http://www.scientistsolutions.com/t13878-mtt+assay.html i just knw 570 nm for test and 630 nm for reference.. anyone xplain me clearly plz]]> Sat, 06 Feb 2010 06:53:48 GMT http://www.scientistsolutions.com/t13877-protein+desalting+and+concetration.html I am working with small amounts of proteins, i have been extracting the protein using the phenol/ ammonium acetate precipitation, after dialysis it becomes hard to recover the protein from the dialysis tube, can someone suggest another way of desalting.
thanks]]> Sat, 06 Feb 2010 06:34:02 GMT http://www.scientistsolutions.com/t13876-callibration+frequency.html I am using UV Spectrophotometer and am calibrating this instrument every month as per the pharmacopoieal requirement ,
and my question is can i change the frequency of the calibration from every month to quarterly ? (as the instruments usage is very les).
To do this what I have to do?( How can i justify my change).
regards
Prabhakar
]]> Sat, 06 Feb 2010 04:05:20 GMT http://www.scientistsolutions.com/t13875-m_tech+with+isim%2c+university+of+mysore+_+admissions+open.html Admissions are now open under Cycle 1 for a multidisciplinary M.Tech – 2010-2012 Programme in Information Systems and Management offered by the International School of Information Management (ISiM), University of Mysore in collaboration with some of the leading Information Schools across the globe - Dalhousie University, Canada; University of Michigan, USA; Univer...]]> Sat, 06 Feb 2010 03:18:30 GMT http://www.scientistsolutions.com/t13874-proteomics.html i was reading some publication on DIGE, and the principle of the tehnique involves labelling two different proteins using cy3 and cy5. To my understanding, only separating two pools of proteins in one gel, y question is what if one wants to seperate several pools of proteins? how do you label them.
thanks]]> Sat, 06 Feb 2010 02:27:59 GMT