http://www.scientistsolutions.com/ Latest discussion from ScientistSolutions.com Life Science Discussion en-us sci7feed@sci7.nojunkorherepleasespam.com hourly 1 2005-05-05T12:00+00:00 http://www.scientistsolutions.com/t14127-vivo_morpholinos+in+murine+kidney+explants.html Thu, 11 Mar 2010 14:54:21 GMT http://www.scientistsolutions.com/t14126-poly_l_lysine+stock+solution.html I have to resuspend poly-L-lysine powder, but I'm a bit confused because I read different protocols.
Should I resuspend it in water or in other buffers?
Thanks]]> Thu, 11 Mar 2010 12:27:23 GMT http://www.scientistsolutions.com/t14125-environmental+control+technician+_+cleveland+oh.html Temporary Duration: Contract
Hours: 1st Shift or 2nd, may need to work Saturdays or Sundays
Pay: $12.00-$13.00
Metro Area - Cleveland - OH USA
City - Bedford
Start: Immediate
Job Description/ Requirements:
The Environmental Monitoring Technicians perform all duties associated with surface and air sampling, associated with personnel during active fill and routine monitoring.
Environmental monitoring is utiliz...]]> Thu, 11 Mar 2010 12:24:00 GMT http://www.scientistsolutions.com/t14124-creating+a+good+seal+in+acutely+isolated+neurons.html I am having a lot of difficulty lately patching acutely isolated neurons from CA1 region of hippocampus. I have no trouble with sealing and breaking through, the problem arises when I lift the cell (after breakthrough) off the plate. Shortly after lifting the cell into the stream of solution, the cell falls off the electrode. I wonder if the problem has to do with the seal itself, the osmolarity of my solutions, CsF in my ICF....
The ECF of my solutions is usually...]]> Thu, 11 Mar 2010 11:56:10 GMT http://www.scientistsolutions.com/t14123-detection+of+free+chlorophyll.html I want to know the methods of detection of free chlorophyll. I want to differentiate between bound and free chlorophyll molecules. Please send me some chemical method by which we can do. Is it possible with the help of HPLC than give me the proper reference? I have small amount of sample.
Thanks in Advance ]]> Thu, 11 Mar 2010 09:02:24 GMT http://www.scientistsolutions.com/t14122-detection+of+free+chlorophill.html I want to know the methods of detection of free chlorophyll. I want to differentiate between bound and free chlorophyll molecules. Please send me some chemical method by which we can do. Is it possible with the help of HPLC than give me the proper reference?
Thanks in Advance ]]> Thu, 11 Mar 2010 08:56:40 GMT http://www.scientistsolutions.com/t14121-how+to+make+a+collagen+iv+gel_.html 96well plate were coated with 400nm of type IV collagen in a total volume of 50ul, incubated for 1hour at 37C to allow the collagen to reconstitute, and then left overnight at 4C.
r2:
8um transwell filters were coated with 100ul of a 100ug/ml collagen IV solution, which was allowed to gel for 1hour at 37C.
Just as two references above, I just want to know what's the difference between "coat collagen directly" and "make collagen gel"?
Can anybody give m...]]> Thu, 11 Mar 2010 06:03:25 GMT http://www.scientistsolutions.com/t14120-propionic+acid+analysis.html how is every one
could you plz.. help me with the analysis of propionic acid on DB-FFAP - GC/FID
that the concentration of the acid is not accurate (area under the peak) for the same concentration after several injection, ghost peak also their, eak saph,
i tried using formic acid between runs, blank, dw, ......
at the column: methanol, ethanol, ACN, propionic acid have the same retion time i dont know how, why, ....
plz. help]]> Thu, 11 Mar 2010 03:00:01 GMT http://www.scientistsolutions.com/t14119-fret+studies+of+gaba+transporter+function+_amp%3b+oligomerization+state.html GABA transporter function, oligomerization state, and anchoring: correlates with subcellularly resolved FRET.
Moss FJ, Imoukhuede PI, Scott K, Hu J, Jankowsky JL, Quick MW, Lester HA.
Division of Biology and 1,2 Program in Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA.
The mouse gamma-aminobutyric acid (GABA) transporter mGAT1 was expressed in neuroblastoma 2a cells. 19 mGAT1 designs incorporating fluorescent proteins we...]]> Thu, 11 Mar 2010 00:10:31 GMT http://www.scientistsolutions.com/t14118-marcello+malpighi.html Wed, 10 Mar 2010 20:06:42 GMT http://www.scientistsolutions.com/t14116-nanodrop+or+real+time+pcr+quantification+of+cdna+for+cox2+gene.html ]]> Wed, 10 Mar 2010 12:44:52 GMT http://www.scientistsolutions.com/t14115-what+are+the+frequently+asked+questions+during+a+research+oral+defense_.html PLEASE! HELP ME PLEASE!
]]> Wed, 10 Mar 2010 09:54:28 GMT http://www.scientistsolutions.com/t14114-fungal+isolates+from+the+air+conditioner+filters.html
My research mates & I have been having problems on our research about "A Study of the Fungal Isolates from the Air Conditioner Filters of the ICU, DR, LR and Nursery of a private hospital and the ICU and DR of a government hospital in _______.

My research mates & I would like to seek help from you.

If you are one of the committee who will ask questions what are the frequently asked questions during a research oral defense?
&...]]> Wed, 10 Mar 2010 08:18:44 GMT http://www.scientistsolutions.com/t14113-science+is+uncertain_.html http://www.toda...]]> Wed, 10 Mar 2010 07:58:31 GMT http://www.scientistsolutions.com/t14112-lab+bratz+episode+%23208.html Wed, 10 Mar 2010 07:49:23 GMT http://www.scientistsolutions.com/t14110-primers.html I am working on lantana camara for cheacking its antibacterial activity on different bacteria.I got great result of antimicrobial activity and i found that antibacterial activity is because of some flavonids present in its leaves.So i want to amplify the gene which is responsible for production of that antibacterial compounds.I am not getting any idea so please help me out.
Regards.
Phani Kumar.
]]> Wed, 10 Mar 2010 04:50:22 GMT http://www.scientistsolutions.com/t14109-free+hrp+in+hrp+conjugated+igg+mab.html Should i try a C18 column and run a RP chromotography with a gradient?
What do you guys recommend, also i have seen some salt deposits on my flow cell, i cle...]]> Tue, 09 Mar 2010 21:24:05 GMT http://www.scientistsolutions.com/t14108-problem+with+methane+analysis.html GC-2010/ Flame Ionizing Detector
Column Temp: Isolthermal 80 ºC
Injection: 200 ºC
Detecter: 200 ºC
He, N2, air zero and H2
When GC is ready for analysis, I inject my sample (methane gas) into injection port and then press "start". Normally (when I tested for agricultural chemical dissloved in solvent like haxane), its status will turn to "acquired" until stop time is reached.
Bu...]]> Tue, 09 Mar 2010 14:34:27 GMT http://www.scientistsolutions.com/t14107-curing+virus_infected+cultures+with+peptide_morpholino+conjugates.html Tue, 09 Mar 2010 12:51:49 GMT http://www.scientistsolutions.com/t14106-gc+solution+software+for+gc_2010.html I can't find this software on the internet. Can't anyone find it on any website? Please tell me. It's urgent!!!!
Thank you for your kindness!]]> Tue, 09 Mar 2010 12:42:12 GMT