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The Sanger Institute gives a great description of how to create a primers, using the Vega Database, or the RepeatMasker, or using Primer3.

List Of Protocols
ExoSeq -ExoSeq Primer Design
ExoSeq Protocols - Pre-Screening
Generating Recombinant Adenovirus
RACE Protocol - Gene Trap Insertion
ExoSeq Protocols - Standard ExoSeq DNA Panel Preparation
PCR - Polymerase Chain Reaction Protocol
DNA 5 Prime End Labeling System
ExoSeq Protocols - Exon Sequencing
AFLP- Amplified Fragment Length Polymorphism
Ethidium Bromide assay for complex formation
Lentiviral Production
Oligonucleotide Arrays - Improved Print and QC Methods
Glycerol Stock and Plasmid Preparation
Lentiviral Infection
ExoSeq Protocols - Standard ExoSeq PCR
PCR - Colony Screening by PCR
Gene Delivery - Retroviral infection of primary human keratinocytes -PHKs
Raft Culture Media - Greens Media
E. Coli Genome Resources
Yeast Genetic Protocols - High Efficiency Transformation from Geitz Lab
Gene Expression- EST- Expressed Sequence Tag
Gene Expression- SAGE-Serial Analysis of Genetic Expression
PCR Mutagenesis
Yeast Genetic Protocols - Procedure for generating ts alleles of NUT2
Yeast Genetic Protocols-Yeast Transformation
Yeast Genetic Protocols - Sleazy Plate Transformation of Yeast
Yeast Genetic Protocols - Bgal Liquid Assays
Yeast Genetic Protocols - Bgal Filter Assays
Yeast Genetic Protocols - Liquid Sporulation
Yeast Genetic Protocols - X Gal Agarose Overlay Assay
Yeast Genetic Protocols- PCR to Check Genotyping
Yeast Genetic Protocols - EMS Mutagenesis