Monoclonal antibodies- expression, purification, affinity assays.

Molecular biology- PCR, cloning, trasfection, transformation, clone selection

Cloning of variants of MABs- ScFv etc

Phage display technology for MAB clone screening

Protein expression -in yeast, mammalian and bacterial systems

Cell culture- continuous cell lines

in vitro bioassays for cytokines- Proliferation, cytotoxicity, Reporter gene assays

Qualification and Validation of analytical methods

Antibodies, Antibody-based Protein Detection, Cell / Tissue Culture, Centrifugation, Chromatography, Cloning, Diagnostic Kits, DNA Isolation & Purification, Electrophoresis, GFP Reporter Assays, Hybridoma, Immunoassays, Immunohistochemistry, Microbiological Cultures, Nucleic Acid Hybridization, Nucleic Acid Labeling, PCR / RT-PCR, Plasmid Isolation & Purification, Protein Purification & Separation, Real Time PCR / RT-PCR, Recombinant Protein Expression & Purification, Transfection, Transformation

Centrifuge, Chromatography, HPLC, Confocal Microscope, Electrophoresis Equipment, Fluorescence Spectrophotometers, High-Throughput Screening Equipment, Incubators, Lab Automation / Robotics, Liquid Handling Systems, Microplate Reader, Multi-Channel Pipettors, Optical Microscope, Orbital Shakers, PCR Kits, PCR Thermocycler, Vacuum Equipment, Vortexing Equipment

Basic Research, Bioanalytical Chemistry, Biochemistry, Biology, Biostatistics, Biotechnology, Cell Biology, Clinical Diagnostics, Computational Biology, Contract Laboratory Services, Diagnostics, Genetic Engineering, Immunology, In Vitro Diagnostics, Laboratory Design, Microbiology, Molecular Biology, PCR, Quality Control / Quality Assurance, Sterilization, Disinfection, & Preservation