My Doctoral studies focused on protein-protein interactions of the aryl hydrocarbon receptor (AHR) with the aryl hydrocarbon receptor nuclear translocator (ARNT, HIF-1B) 1 and 2 and those of ARNT1 and ARNT2 with the hypoxia-inducible factor (HIF-1a).  Studies also examined the mechanism of degradation of the AHR, which occurs following ligand binding.

My postdoctoral research focuses on examining co-activators and co-repressors implicated in altering the dose response of the glucocorticoid receptor.

Antibody-based Protein Detection, Cell / Tissue Culture, Cell Signaling Assays, Centrifugation, Cloning, DNA Isolation & Purification, Electrophoresis, GFP Reporter Assays, Immunoassays, Microbiological Cultures, PCR / RT-PCR, Plasmid Isolation & Purification, Protein Purification & Separation, Protein-Nucleic Acid Interaction Analysis, Protein-Protein Interaction Analysis, Real Time PCR / RT-PCR, Recombinant Protein Expression & Purification, RNA Isolation & Purification, RNAi & Gene Silencing Assays, Site-Directed Mutagenesis, Spectroscopy, Fluorescence, Spectroscopy, UV-VIS, Transduction, Transfection, Transformation

Centrifuge, Electrophoresis Equipment, Imaging Systems, Immunoassay Equipment, Incubators, Multi-Channel Pipettors, Optical Microscope, Orbital Shakers, PCR Kits, PCR Thermocycler, Pipettors, Real Time PCR Thermocycler, RNA Interference / Gene Silencing Kits, UV/VIS Spectrophotometers, Vacuum Equipment, Vortexing Equipment, Water Purification Systems

Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Proteomics, Toxicology