I am trying to understand the physiological signoficance of E6AP, a protein the dysfunction of which results in Angelman Syndrome. To this end I am using both invito and mice models to dissect out the pathways affected in AS and, thus characterise the importance of E6AP in normal state.

Antibodies, Antibody-based Protein Detection, Cell / Tissue Culture, Cell Signaling Assays, Centrifugation, Chromatography, Cloning, Cytology Staining, DNA Isolation & Purification, DNA Sequencing, GFP Reporter Assays, Immunoassays, PCR / RT-PCR, Phosphorylation Analysis, Plasmid Isolation & Purification, Protein Purification & Separation, Protein-Protein Interaction Analysis, Real Time PCR / RT-PCR, RNA Isolation & Purification, RNAi & Gene Silencing Assays, Spectroscopy, Fluorescence, Transfection, Transformation

Centrifuge, Chromatography, HPLC, Confocal Microscope, Data Acquisition Equipment, DNA Sequencers, Fluorescence Spectrophotometers, Imaging Systems, Immunoassay Equipment, Incubators, Liquid Handling Systems, Microarray Platforms, Microplate Reader, Orbital Shakers, PCR Kits, PCR Thermocycler, Pipettors, Real Time PCR Thermocycler, RNA Interference / Gene Silencing Kits, UV/VIS Spectrophotometers, Vortexing Equipment, Water Purification Systems

Anatomy, Basic Research, Biochemistry, Biology, Cell Biology, Genetics, Immunology, Molecular Biology, Neuroscience, PCR, Physiology, Proteomics, RNA Interference / Gene Silencing, RT-PCR