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Personal Information
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Ilan Eliassi, M.Sc. M.B.A Manager of Assay Development and New Technologies
Frog Egg
Rehovot, Israel
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I am Ilan Eliassi, M.Sc. M.B.A Manager of Assay Development and New Technologies working in .
My Blog:
www.scientistsolutions.com/blog/eliassi
My Website:
www.proteologics.com
About My Research
As a manager of new technologies and assay development and Senior scientist as well as projects leader in the Ubiquitin field
I'm Working on various projects involving the Ubiquitin re-cycling system for Amino Acids (in association with big Pharma companies in ISRAEL and abroad).
My wrok involves planning of milestones in projects, building, adaptation and validation of ISO compatible protocols. Fine tuning and optimization of assay terms, Quality assurance and Quality control (QA/QC), developing SOPs based on GLP&GMP (controlled documents) and adaptation to robotics unit (HTS). Rich experience in QC, QA, development of protocols and SOPs supporting GMP & GLP.
Development and delivery of protocols to HTS group in order to search and trace compounds, among known compound libraries, that inhibit specifically target proteins. Those compounds were checked to be effective, non cyto-toxic (in cell assays as well as animal studies).
The work includes qualification of HTS and R&D teams to work, based on SOPs.
In parallel I participated in practice research, combining planning and performing cell based and/or cell free (recombinant purified Proteins, manufactured at our site or commercial) screening assays (purified recombinant Proteins, ectopically expressed in mammalian cell lines).
Various Enzyme studies (define Km, V0, Vmax, and KI).
The experiments utilized many different analytic techniques such as: ELISA, FACS, ECL HRP assay, Chromogenic HRP assays, HTRF, Fluorescence Polarization, Fluorescence Intensity, TLC Chromatography, Enzymatic assays using precursors labeled with Radioactive Isotopes (C 14)… Molecular Biology ( DNA, RNA and Proteins), Cell culture (cells grown on adhesive Polypropylene matrix and in suspension), Virology (Infections), Immunology, Ligand-Receptor binding, induction, differentiation.
The research studies involve research combined with planning experiments in Vitro (cell based and cell free) and in Vivo (animal models).
Lab Techniques
Antibodies, Antibody-based Protein Detection, Cell / Tissue Culture, Cell Signaling Assays, Centrifugation, Chromatography, Cloning, Cytology Staining, Diagnostic Kits, Data Processing, DNA Isolation & Purification, Drug Development, Electrophoresis, Fermentation, Filtration, Flow Cytometry, Gene Amplification, GFP Reporter Assays, Hybridoma, Immunoassays, Immunohistochemistry, Lyophilization, Microbiological Cultures, PCR / RT-PCR, Phosphorylation Analysis, Plasmid Isolation & Purification, Protein Purification & Separation, Protein Synthesis, Protein-Nucleic Acid Interaction Analysis, Protein-Protein Interaction Analysis, Real Time PCR / RT-PCR, Recombinant Protein Expression & Purification, RNA Isolation & Purification, RNAi & Gene Silencing Assays, Signal Processing, Spectroscopy, FCS, Spectroscopy, Fluorescence, Transduction, Transfection, Transformation, Viral Vector Assays
Research Focus
Basic Research, Biochemistry, Biology, Biomedical Technology, Biotechnology, Cancer, Cell Biology, Data Analysis Tools, Diagnostics, Discovery Informatics, Drug Discovery, Gene Expression Analysis, Genetics, Genomics, H-T Screening, Immunology, In Vitro Diagnostics, Material Science, Medical Device Development, Medicine, Microbiology, Molecular Biology, PCR, Pharmacology, Protein Chemistry, Proteomics, Quality Control / Quality Assurance, RNA Interference / Gene Silencing, RT-PCR, Sterilization, Disinfection, & Preservation, Toxicology, Virology
Other Interests & Activities
1. Zusman, T., O. Gohar, I. Eliassi, Y. Avivi, E. Lisansky, C. Sautès, J. Even, C. Bonnerot, W.H. Fridman, I.P. Witz and M. Ran, The Murine Fc-Gamma (Fcγ) Receptor type II B1 is a Tumorigenicity-Enhancing Factor in Polyoma-virus-Transformed 3T3 cells. Int. J. Cancer, 1996. 65: 221-229.
2. Ran, M., A. Ben-Baruch Langer, I. Eliassi, O. Gohar, B. Gonen, S. Gradsztajn, W.H. Fridman, J.L. Teillaud and I.P. Witz, Possibilities of Interference with the Immune System of tumor bearers by Non-Lymphoid FcγRII Expressing Tumor cells. Immunobiol., 1992. 185:415-425.
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