The major focus of my laboratory is in diabetes and how this disease affects heparan sulfate proteoglycan formation and lipid metabolism. These studies can uncover concepts that directly impact human disease as altered metabolism of lipids in Type 1 Diabetes can lead to cardiovascular disease, the major cause of mortality in these patients.

 

Antibodies, Antibody-based Protein Detection, Cell / Tissue Culture, Cell Signaling Assays, Centrifugation, Cytology Staining, DNA Isolation & Purification, DNA Sequencing, Electrophoresis, Flow Cytometry, Mass Spectroscopy, Microbiological Cultures, Nucleic Acid Hybridization, Nucleic Acid Labeling, PCR / RT-PCR, Plasmid Isolation & Purification, Protein Purification & Separation, Protein Synthesis, Protein-Protein Interaction Analysis, Real Time PCR / RT-PCR, Recombinant Protein Expression & Purification, RNA Isolation & Purification, RNAi & Gene Silencing Assays, Transfection, Transformation

Centrifuge, Chromatography, HPLC, Confocal Microscope, Computers (IT equipment), Electron Microscope, Filtration Systems, Fluorescence Spectrophotometers, Freeze Dryers, Imaging Systems, Immunoassay Equipment, Incubators, LC, LCMS, Liquid Handling Systems, Mass Spectrometry, MALDI-TOF, Microplate Reader, Multi-Channel Pipettors, Optical Microscope, Orbital Shakers, PCR Kits, PCR Thermocycler, Pipettors, Real Time PCR Thermocycler, RNA Interference / Gene Silencing Kits, UV/VIS Spectrophotometers, Vacuum Equipment, Vortexing Equipment

Basic Research, Biochemistry, Cell Biology, Medicine, Molecular Biology, Protein Chemistry, Proteomics, RT-PCR

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